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Bee's knees (sort of....) Part II

 
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Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Wed Apr 13, 2005 8:47 pm    Post subject: Bee's knees (sort of....) Part II Reply with quote

After I took a few low mag shots of the rear leg of a honeybee I was curious what some of the parts would look like a little "closer up".

It's pretty obvious why they call these the "pollen combs"



And the auricle, part of the "pollen press" in the joint of the basitarsus and tibia, is very interesting when viewed at a little higher magnification. Looks like the perfect tool for the job of crushing and packing pollen. Amazing when you think about it. (Context for the location of this "part" can be seen in the previous "bee's knees" post)


These two shots were taken using a 10X objective, and were illuminated with fiber optic light guides. This is a Nikon Plan, and is the highest mag objective I have that can be illuminated easily from above. Both images are from "stacks" combined using Helicon Focus.
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piotr



Joined: 28 Apr 2004
Posts: 445
Location: San Diego

PostPosted: Wed Apr 13, 2005 9:06 pm    Post subject: Reply with quote

Absolutely amazing!!!

The pollen grains look very beautiful. Do you have any idea what kind of plant this pollen comes from? Maybe canola (Brassica rapa)?

Charlie, I think these pictures are much better than SEM! I find this bee series extremely interesting. My dad's hobby is beekeeping, I'm going to show him your pictures.
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Charles Krebs



Joined: 13 Jul 2004
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Location: Issaquah, WA USA

PostPosted: Wed Apr 13, 2005 9:15 pm    Post subject: Reply with quote

Piotr... Thanks! The pollen is from Camellia blossoms.
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Ken Ramos
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Joined: 04 May 2004
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PostPosted: Thu Apr 14, 2005 2:56 am    Post subject: Reply with quote

These are some excellent image Charlie. The detail and color are outstanding. I especially like the 3D effect they have. Thanks and keep up the Bee series if you can. It would be interesting to see more. Very Happy
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Dembowski



Joined: 12 Feb 2005
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Location: Pennsylvania, USA

PostPosted: Thu Apr 14, 2005 6:24 am    Post subject: Reply with quote

O my goodness ... they're beautiful.
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Wim van Egmond



Joined: 08 Apr 2005
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Location: Rotterdam, the Netherlands

PostPosted: Thu Apr 14, 2005 8:40 am    Post subject: Reply with quote

Wonderful images! This technique is so great. I think you will not make any other type of macro image! Smile

Wim
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Charles Krebs



Joined: 13 Jul 2004
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Location: Issaquah, WA USA

PostPosted: Thu Apr 14, 2005 10:02 am    Post subject: Reply with quote

Appreciate the nice comments... Cool . "Stacking" certainly is a new and fun toy ... uhhh.... I mean tool! (I do feel like a kid with a new toy Wink) Still trying to sort out what subjects work well, and which do not. Also trying to figure out the best lighting and shooting methods. But there is no question that for many static "3D" subjects it's a valuable technique.

Subjects like this were so tough to photograph because you were so limited as to your shooting angle because of DOF reasons. Sometimes the required shooting angle was not the most informative or aesthetic. Viewing with the microscope, you are constantly (and unconsciously) moving focus and assembling a complete "picture" of the subject in your mind. The camera can't do that, and from a "psychological" point of view, seeing a photo with only a very narrow band that is in focus can, at times, be frustrating. So I look at this technique as accomplishing for the camera, the image "assembly" we do mentally by varying the fine focus when observing the specimen.
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nephiliim



Joined: 23 Mar 2005
Posts: 546

PostPosted: Thu Apr 14, 2005 11:09 am    Post subject: Reply with quote

How do you make stackings? Do you make layer photo's of different focus levels and then put them together? Seems like a lot of work to me...

But the result is great man, even my brain sometimes can't handle a microscopic subject this clearly.
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Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Thu Apr 14, 2005 12:59 pm    Post subject: Reply with quote

Tom.... yup... basically you take several (many?) photos moving the focus slightly through you subject. You then combine them. Check out this topic:
http://www.photomacrography1.net/forum/viewtopic.php?t=1794
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