The two images above were both taken using a Wild M8 microscope at different zoom settings. Both are stacks combined using Helicon Focus.
These lower images show the effect of changing the mounting medium. The first image, taken in propan-2-ol (isopropanol or isopropyl alcohol) was a single image, the one below a stack of 16 with the specimen in water. I also tried immersion oil. This dissolved the yellow colouring of the pollen, leaving yellow haloes around the grains. Microscope was a Zeiss Standard GFL with Zeiss x40 plan achromatic objective and Watson x8 compensating eyepiece.
Attempts to get some intermediate magnification images using image stacks from the Projectina microscope produced yellow fog in both Helicon and CombineZ.
These pollen grains are like miniature conkers (horse chestnuts to those not versed in the ways of British schoolboys).
