Do you think these are contractile-vacuole pores?
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Do you think these are contractile-vacuole pores?
I was watching contractile vacuoles on some paramecium bursaria and noticed that when I shifted focus from the vacuole inside the cell to the cells surface there were always two rounded "ports" in the pellicle directly above the vacuole.
In "1a" you can see the two circular structures on the surface, and in "1b" I have quickly focused "into" the cell to show the full vacuole directly underneath. "2a" and "2b" show the same thing on a different paramecium. I checked about 4 or 5 individuals and found the same.
The inset in the lower left shows these structures with a scale bar. (Lower right shows a whole paramecium bursaria.)
I know you are supposed to be able to see the pores that empty the vacuole contents, but I've never seen them in a picture, and I've never read about two pores for a vacuole. Do you all think this is what these are?
1a, 1b, 2a, 2b, lower left inset taken with Olympus 100X, 3.3X photo-eyepiece, Canon 10D.
Whoa! Talk about a detailed image! I would guess that, that is what they are Charlie. I have read about them in various articles concerning water expelling vesicles (wev) but have never seen them until now. I would imagine some protozoologist somewhere would like very much to see these. Excellent work here indeed.
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Kenneth Ramos
Rutherfordton, North Carolina
Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
Kenneth Ramos
Rutherfordton, North Carolina
Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
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Steve... yes these are "slightly" oblique brightfield... fairly small offset. (Except for the full critter shot, which was "rheinberg-oblique"). I really like oblique-brightfield. The biggest problem is that if you go very "strong" oblique you lose color, but you will pick up detail you can't see without it.
Charlie, what a wonderful gallery ! I never saw vacuoles emptying so detailed. And i never saw two pores.
That is a question, Prof. Hausmann in Berlin could answer : hausmann@zedat.fu-berlin.de
He is protozoologist and a very kind person, publisher of the Mikrokosmos, the leading
magazine in microscopy in Germany.
Gerd
That is a question, Prof. Hausmann in Berlin could answer : hausmann@zedat.fu-berlin.de
He is protozoologist and a very kind person, publisher of the Mikrokosmos, the leading
magazine in microscopy in Germany.
Gerd
Hello Charlie,
Do you have any proof that you are from this planet? Your images are 'other worldly' and I figure you are using some alien technology that hasn't been discovered by us Earthlings!
I haven't commented on a lot of the shots you have posted, but please allow me to add my plaudits to the others; well deserved they are.
You have set the benchmark, and then keep raising it! Great work, and as one of the other people mentioned, the level of images being presented on this forum are without equal, and done by "amatuers."
Some amatuers!
All the best,
Mike
Do you have any proof that you are from this planet? Your images are 'other worldly' and I figure you are using some alien technology that hasn't been discovered by us Earthlings!
I haven't commented on a lot of the shots you have posted, but please allow me to add my plaudits to the others; well deserved they are.
You have set the benchmark, and then keep raising it! Great work, and as one of the other people mentioned, the level of images being presented on this forum are without equal, and done by "amatuers."
Some amatuers!
All the best,
Mike
Charlie,
Excellent images and pro-level reaearch!
Here I found a following excerpt in the "Biology of Paramecium" book by Ralph Wichterman (The Blakitson Company, 1953, p. 35):
"(...) Kalmus (1931) reports that he has at times been able to observe two pores at one contractile vauole in P. caudatum and King (1935) has seen two in P. aurelia. Kahl (1930) is of the opinion that P. bursaria also possesses two excertion pores for each vacuole."
Excellent images and pro-level reaearch!
Here I found a following excerpt in the "Biology of Paramecium" book by Ralph Wichterman (The Blakitson Company, 1953, p. 35):
"(...) Kalmus (1931) reports that he has at times been able to observe two pores at one contractile vauole in P. caudatum and King (1935) has seen two in P. aurelia. Kahl (1930) is of the opinion that P. bursaria also possesses two excertion pores for each vacuole."
Piotr
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