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Water Boatman Leg (from 11 images stacks)

 
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Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Thu Jun 09, 2005 9:59 am    Post subject: Water Boatman Leg (from 11 images stacks) Reply with quote

Last spring I became interested in the structure of the leg of the Water Boatman (Hemiptera:Corixidae). They have a surprising number of specailized features, including one section that has flattened hairs that function and pivot like many oars. (Seeing this, it becomes obvious why they are such powerful swimmers).

Depth of field was frustrating with such a subject, so I decided to take another look now that image stacking software is readily available. (Last year I laboriously combined images manually in Photoshop)



The above image was taken with a 40X objective, and 11 frames were combined using Helicon Focus. I'm not sure of the purpose of these rounded hairs... I think they might be used to hold air bubbles against the leg... need to observe some active specimens more closely!



This image was taken with a 100X objective and is also combined from a stack of 11 images. In this image, the specialized, flat "oar" hairs and their interesting moving joint are clearly seen.

(Some of the shots I took last year can be found here:http://micropix.home.comcast.net/waterb/index.html These were more colorful, since I used Rheinberg filters. I think I'll dig out the Rheinberg filters again, and together with Helicon Focus, see what I can do with both techniques combined)
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Gyrosigma



Joined: 22 Dec 2004
Posts: 73
Location: France (Normandy)

PostPosted: Thu Jun 09, 2005 11:40 am    Post subject: Reply with quote

Surprised
I have not enough English vocabulary to say what feeling I have Charles....

Very impressive....

best regards

Dominique
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gpmatthews



Joined: 27 Apr 2004
Posts: 539
Location: Horsham, UK

PostPosted: Thu Jun 09, 2005 1:09 pm    Post subject: Reply with quote

Charlie - breathtakingly wonderful images!
Absolutely great!
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Graham
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Dembowski



Joined: 12 Feb 2005
Posts: 44
Location: Pennsylvania, USA

PostPosted: Thu Jun 09, 2005 3:31 pm    Post subject: Reply with quote

Great images, Charlie. There's more going on here than just the use of HF. You do good stuff.
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Bill Dembowski
"It's alive .... It's alive !!!!
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piotr



Joined: 28 Apr 2004
Posts: 445
Location: San Diego

PostPosted: Fri Jun 10, 2005 12:10 pm    Post subject: Reply with quote

Absolutely stunning. I like the first image very much, exposure is perfect. It has this 'real thing' feel. All other pictures are wonderful, too.
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Piotr
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nephiliim



Joined: 23 Mar 2005
Posts: 546

PostPosted: Fri Jun 10, 2005 1:49 pm    Post subject: Reply with quote

That's it, I'm gonna ask if I can borrow a microscope from my school this summer!!!!



Wow!!!! This (and the the external site images) are just wonderfull charles, The detail is great.

Do you fixate the focus on your camera? What kind of camera did you use? How much zoom does one need to get past the tubus of the microscope?

All the best,

Tom
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Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Sat Jun 11, 2005 12:16 pm    Post subject: Reply with quote

Thanks all!

Tom... I use a Canon 10D DSLR. You can get a pretty good idea of how I am set up here.
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lacerta



Joined: 12 Feb 2005
Posts: 139
Location: Georgia, USA

PostPosted: Sat Jun 11, 2005 9:04 pm    Post subject: Reply with quote

Incredible images. Also interesting web page article on your set up. I really appreciated the description. It gives me some food for thought as I began to get things set up here. Thanks Charles.
George
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Wim van Egmond



Joined: 08 Apr 2005
Posts: 440
Location: Rotterdam, the Netherlands

PostPosted: Mon Jun 13, 2005 3:33 am    Post subject: Reply with quote

It is beautiful Charlie!

About teh function of these hairs. Years ago I made a little article about what can be found on a spider's leg. www.microscopy-uk.org.uk/mag/artjan00/spileg.html

Some of these hairs are quite similar and have sensory functions.

Wim
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