Hollyhock killers in our garden (Hollyhock Rust, Puccinia ma

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Gerd
Posts: 160
Joined: Wed Feb 16, 2005 2:44 pm
Location: near Cologne, Germany

Hollyhock killers in our garden (Hollyhock Rust, Puccinia ma

Post by Gerd »

Image

Rost is the most common disease of Hollyhock in our gardens. Little yellow spots on the
leaf surface are associated with the spore pustules on the lower leaf side, where the spores were produced.
This is a hand section through a hollyhock leaf in the centre of that spore pustule.
On the slide the section was squeezed a little to show the spores better.
Objective 20 x , DIC
Thanks,
Gerd

Charles Krebs
Posts: 1200
Joined: Tue Jul 13, 2004 10:50 am
Location: Issaquah, WA USA

Post by Charles Krebs »

Gerd... another beautifully prepared and photographed specimen. What equipment do you use to cut your hand sections?

lacerta
Posts: 139
Joined: Sat Feb 12, 2005 10:16 am
Location: Georgia, USA

Post by lacerta »

Yes, how do you manually cut your sections??? Those are great images.
George

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Gerd
Posts: 160
Joined: Wed Feb 16, 2005 2:44 pm
Location: near Cologne, Germany

Post by Gerd »

Image

Hi George, Hi Charlie,
thanks for the compliments ! Here is my howto :
until the sunflower petal section i worked the usual manual way, shown i the first
part of the image. Some days ago, i got a little tool from a friend of mine,
this little knife holder is a wonderful tool to work with a hand microtome.
My previous experience in using this hand microtome was very frustrating,
because the blades quickly blunt when scratching on the glass plate.
Additionally the disposable microtome blades i use are rather narrow
in handling with the microtome table plate.
The "secret" of this knife holder is on the lower side, there is a cut-out for
the sectioning object, so the blade floats over the object.
Thanks,
Gerd

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