:: View topic - Honeybee eye
 Forum Index
An online community devoted to the practices of photomacrography and amateur microscopy..
 
New! Photomacrography2.net
 
This site is no longer available for posting or registering new members.
 FAQFAQ   SearchSearch     UsergroupsUsergroups   
 ProfileProfile   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Honeybee eye

 
This forum is locked: you cannot post, reply to, or edit topics.   This topic is locked: you cannot edit posts or make replies.     Forum Index -> Photography Through the Microscope Gallery
View previous topic :: View next topic  
Author Message
Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Sat Sep 17, 2005 12:10 am    Post subject: Honeybee eye Reply with quote

Haven't been able to post much lately, but I did find enough time to combine 87 shots with Helicon Focus to get the depth on this honeybee eye.




10X objective, 2.5X photo-eyepiece. Canon 350D. Two fiber optic light guides through small diffusion "dome".
Back to top
View user's profile Send private message Send e-mail
Mike



Joined: 12 Dec 2004
Posts: 217
Location: Northeast Ohio, USA

PostPosted: Sat Sep 17, 2005 10:54 am    Post subject: Reply with quote

Hello Charlie,

Wonderful shot that will likely be used to teach biologists! (If not, it should be.)

I'm a little taken aback by 87 shots - is that number needed?

All the best,

Mike
_________________
"Nil satis nisi optimum"
Back to top
View user's profile Send private message
Ken Ramos
Site Admin


Joined: 04 May 2004
Posts: 4809
Location: Western North Carolina

PostPosted: Sat Sep 17, 2005 11:13 am    Post subject: Reply with quote

This is a dang good shot Charlie. Very Happy Do you think you could have gotten a little more detail in it though? Laughing
_________________
Site Admin.
Kenneth Ramos
Rutherfordton, North Carolina
Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
Back to top
View user's profile Send private message
Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Sat Sep 17, 2005 12:29 pm    Post subject: Reply with quote

Mike... 87 might be a little overkill Wink
But I used a 10X with a pretty high na so DOF was really shallow. And any time the subject has fine hairs protruding toward the objective like this I like to err on the side of too many images. Otherwise you might "miss" a layer and the software can't make a continuous straight line out of the hair, and you get a jagged line.
In addition, it's hard to tell from the picture, but the antenna protruded closer to the lens than the eye, so a significant number of images were needed just to "pull" that into focus.

If I have some time, maybe I'll run it again with every other image in the stack and see how much difference there is.
Back to top
View user's profile Send private message Send e-mail
Ken Ramos
Site Admin


Joined: 04 May 2004
Posts: 4809
Location: Western North Carolina

PostPosted: Sat Sep 17, 2005 2:02 pm    Post subject: Reply with quote

Hey Charlie, what is that cup shaped object just above the eye and just to the right of it? Any ideas? Very Happy
_________________
Site Admin.
Kenneth Ramos
Rutherfordton, North Carolina
Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
Back to top
View user's profile Send private message
Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Sat Sep 17, 2005 2:27 pm    Post subject: Reply with quote

Ken... That's an ocellus. It's a little tough to get oriented on this shot because the heads is at a strange angle... but it's one of the "simple" eyes on top of the head.
Back to top
View user's profile Send private message Send e-mail
lacerta



Joined: 12 Feb 2005
Posts: 139
Location: Georgia, USA

PostPosted: Sat Sep 17, 2005 9:06 pm    Post subject: Reply with quote

Another incredible image! As always I am inspired. Your comments on technique have taught me much. I am obsessed with detail and focus and so far my images have been rather disappointing. 87 images!? Hmmm. Maybe that's where I can improve. Instead of taking quarter-turns on the fine focus knob, perhaps smaller "bites" will help. Thanks Charlie.
George
Back to top
View user's profile Send private message
Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Sat Sep 17, 2005 10:15 pm    Post subject: Reply with quote

Mike... I played around with the stack and you were right. This could probably have been made with about 25 images. I've been frustrated a few times when I made the interval too large and encountered problems, so I really overdo it sometimes!

So George.... nevermind the 87!... save yourself some hard disk space. Wink
Back to top
View user's profile Send private message Send e-mail
Ken Ramos
Site Admin


Joined: 04 May 2004
Posts: 4809
Location: Western North Carolina

PostPosted: Sun Sep 18, 2005 3:21 am    Post subject: Reply with quote

Charlie said:
Quote:
Ken... That's an ocellus. It's a little tough to get oriented on this shot because the heads is at a strange angle... but it's one of the "simple" eyes on top of the head.


I sort of thought so Charlie but I am used to seeing them farther back from the eye of most insects and that it appeared "cup" shaped to me. That is what prompted my question. Thanks Charlie. Very Happy
_________________
Site Admin.
Kenneth Ramos
Rutherfordton, North Carolina
Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
Back to top
View user's profile Send private message
Display posts from previous:   
This forum is locked: you cannot post, reply to, or edit topics.   This topic is locked: you cannot edit posts or make replies.     Forum Index -> Photography Through the Microscope Gallery All times are GMT - 7 Hours
Page 1 of 1

 
Jump to:  
You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot vote in polls in this forum


Powered by phpBB 2.0.6 © 2001, 2002 phpBB Group