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Olympus HI M100 question

 
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wilash



Joined: 13 Apr 2005
Posts: 104
Location: Japan

PostPosted: Sun Apr 30, 2006 8:51 am    Post subject: Olympus HI M100 question Reply with quote

I found an Olympus HI M100 objective. With a NA of 1.30, I am sure it requires an immersion liquid to work. What would I need, how would I handle it, and what would I use to clean the objective. All my objectives are dry and so this is new to me.
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Last edited by wilash on Sun Apr 30, 2006 9:27 pm; edited 1 time in total
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wilash



Joined: 13 Apr 2005
Posts: 104
Location: Japan

PostPosted: Sun Apr 30, 2006 9:26 pm    Post subject: Reply with quote

OK. I think I can answer a few of my questions:

HI = Homogeneous Immersion

The objective has a black band which indicates oil. But which oil? I take it Castrol GTX, while being liquid engineering, is not the stuff. Or does all oil used in microscopy have the same refractive index?

Any tips about using oil is welcome.
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wilash



Joined: 13 Apr 2005
Posts: 104
Location: Japan

PostPosted: Sun Apr 30, 2006 9:32 pm    Post subject: Reply with quote

(Please excuse me while I have this conversation with myself.)

Here is a good article that may help:

http://www.cargille.com/immersionoilmicroscope.shtml
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rene



Joined: 17 Mar 2005
Posts: 125

PostPosted: Thu May 11, 2006 2:51 pm    Post subject: Reply with quote

Funny conversation Wink

Some oils can be removed with alcohol, but I really would recommend pure white cleaning petrol. Most scope manufacturers would recommend against alcohol! Use little on a soft tissue and wipe the oil off. Saliva works very well also. It is not really necessary to keep the lens spotless, but don't let the oil get contaminated with dust etc. Do not use ceder oil as in the good old times: it hardens!

Good luck, Rene.
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