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Paramecium bursaria, final moment of transverse fission

 
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Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Fri Dec 10, 2004 1:26 am    Post subject: Paramecium bursaria, final moment of transverse fission Reply with quote



These four panels show the "Christmas Paramecium" I posted the other day (http://www.photomacrography1.net/forum/viewtopic.php?t=834) at the final moment of reproducing by fission.

1) Gives an overview where it can be seen that the process is nearly complete.
2) Shows a closer detail at about the same stage as 1
3) The last moment. As the two organisms moved apart, the cell wall stretched sort of like taffy or silly putty
4) The connection suddenly "broke" and here you can see the points of connection as they move toward each organism. Within a second or two, these points were gone and the cell wall looked rounded and smooth.

2, 3, and 4 taken with 40X objective, 3.3X photo-eyepiece. Oblique bright-field with crossed polarizers. Canon 10D. Electronic flash. (1 taken with 20X)
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Ken Ramos
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Joined: 04 May 2004
Posts: 4809
Location: Western North Carolina

PostPosted: Fri Dec 10, 2004 4:45 am    Post subject: Reply with quote

Very Happy Often I have wondered about this final phase of division. I have never been able to see it this clearly until now. Thanks Charlie Very Happy Cool
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Reposts of my images within the galleries are welcome, as are constructive critical critiques.
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Steve West



Joined: 10 Oct 2004
Posts: 545
Location: Tucson, AZ USA

PostPosted: Fri Dec 10, 2004 6:13 am    Post subject: Reply with quote

Absolutely excellent work Charlie! I have to ask a dumb question. Was this the same paramecium you photographed a day or two ago? If so, how did you keep it alive and viewable under the microscope? The detail you captured is spectacular.

Steve
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micron



Joined: 29 May 2004
Posts: 294
Location: Delta, BC - Canada

PostPosted: Fri Dec 10, 2004 9:14 am    Post subject: Reply with quote

Hi Charlie,

You keep raising the bar - outstanding sequence - perfect timing!

All the best,
Ron
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Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Fri Dec 10, 2004 12:17 pm    Post subject: Reply with quote

Steve... It's the same one. These pictures were taken about 20-30 minutes after the one I posted originally. If you take another look at that one (http://www.photomacrography1.net/forum/viewtopic.php?t=834) you can see the division just barely starting. (As that eagle eye Ken pointed out Wink )
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Kenv



Joined: 24 May 2004
Posts: 852
Location: New Zealand

PostPosted: Fri Dec 10, 2004 1:41 pm    Post subject: Reply with quote

Absolutely super Charlie, I guess this is also cross polarisation being used and also flash?
Kenv
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