Diatoms with newly acquired Zeiss x100 plan apo PH3

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gpmatthews
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Diatoms with newly acquired Zeiss x100 plan apo PH3

Post by gpmatthews »

I acquired a new objective on e-bay and these are the first images taken whilst trying it out. It took me nearly a week to find the time - things have been a bit hectic of late, hence the lack of postings. I do not have a substage PH3 phase ring for this lens (or any other), so I have prepared a set of substage patch stops with patches in increments of 1 mm. I choose the one that just fits inside the phase ring of a given phase objective and then close down the substage condenser diaphragm to just graze the outside of the objective phase ring, using a pinhole eyepiece for alignment. The technique works quite well.

Image
Pleurosigma fragment
Zeiss Standard GFL Microscope
Ocular: Olympus P15
Objective: Zeiss x100 Plan Apo PH3
Phase contrast
Camera: Canon Powershot S50
ISO 50, F4.9, 1/3 sec



Image
Centre of centric diatom
Zeiss Standard GFL microscope
Ocular: Olympus P15
Objective: Zeiss x100 Plan Apo PH3
Phase contrast
Camera: Canon Powershot S50
ISO 50, F4.9, 1/3 sec
Slde: Dunkirk (Frez)



Image
Another centric diatom
Zeiss GFL Microscope
Ocular: Olympus P15
Objective: Zeiss x100 Plan Apo PH3
Phase contrast
Camera: Canon Powershot S50
ISO 50, F4.9, 1/3 sec
Slde: Dunkirk (Frez)
Graham

rjlittlefield
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Post by rjlittlefield »

Graham,

Pardon my ignorance, but I'm not sure I understand what you're doing. I have no hands-on experience with phase objectives. I gather that you're using the objective in phase mode, but with a home-brew condenser annulus instead of one designed for the job. Is that right?

Thanks,
--Rik

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gpmatthews
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Post by gpmatthews »

Rik -

That's correct. The method is more flexible than having fixed condenser annuli and of course the substage stops have other uses. A poor man's Heine condenser!
Graham

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piotr
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Post by piotr »

Congratulations on the objective, Graham!

Great pictures, resolution is very decent, indeed. And your home-made phase ring seems to work flawlessly.
Piotr

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Ken Ramos
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Post by Ken Ramos »

Indeed your homemade annulus does work quite well Graham, as does the new objective. Very nice! :D
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Kenneth Ramos
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Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.

Charles Krebs
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Post by Charles Krebs »

Graham... sounds like (and looks like!) a nice objective. Good job on the "home-made" phase condenser...I always enjoy your clever equipment implementations.

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