This is Paramecium stained with nigrosin. A little drop of a concentrated solution of nigrosin is added to a drop of Paramecium culture placed on a slide. The slide is left until the drop dries. Details of pellicle, cillia and cytostome can be demostrated this way. Nigrosin can be replaced with black ink (I haven't tested it, though). I've got the stain from Cynmar last year.
25x/N.A.0.65 objective, 2x Optovar setting, Sony DSC-V1
Paramecium - staining experiment
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- discomorphella
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Piotr--This is a great shot. I especially like how it looks like a low-power EM shot. You might want to try one of the simpler silver protein staining techniques too (you can use silver nitrate / gelatin and sunlight), there are some that aren't that difficult to do, if you want a more transparent preparations (especially background) than the nigrosin.
--David
--David
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