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Paramecium - staining experiment

 
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piotr



Joined: 28 Apr 2004
Posts: 445
Location: San Diego

PostPosted: Wed Apr 06, 2005 12:49 pm    Post subject: Paramecium - staining experiment Reply with quote

This is Paramecium stained with nigrosin. A little drop of a concentrated solution of nigrosin is added to a drop of Paramecium culture placed on a slide. The slide is left until the drop dries. Details of pellicle, cillia and cytostome can be demostrated this way. Nigrosin can be replaced with black ink (I haven't tested it, though). I've got the stain from Cynmar last year.



25x/N.A.0.65 objective, 2x Optovar setting, Sony DSC-V1
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Piotr
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discomorphella



Joined: 06 Jan 2005
Posts: 63
Location: NW United States

PostPosted: Wed Apr 06, 2005 7:17 pm    Post subject: Reply with quote

Piotr--This is a great shot. I especially like how it looks like a low-power EM shot. You might want to try one of the simpler silver protein staining techniques too (you can use silver nitrate / gelatin and sunlight), there are some that aren't that difficult to do, if you want a more transparent preparations (especially background) than the nigrosin.

--David
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piotr



Joined: 28 Apr 2004
Posts: 445
Location: San Diego

PostPosted: Wed Apr 06, 2005 8:44 pm    Post subject: Reply with quote

David, thank you. Yes, I will try the silver staining method - definitely.
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Wim van Egmond



Joined: 08 Apr 2005
Posts: 440
Location: Rotterdam, the Netherlands

PostPosted: Tue Apr 12, 2005 3:37 am    Post subject: Reply with quote

Dear Piotr,

That is very good! Nice colours and the pattern of the arrangement of the cilia is very nice. I hope you succeed in finding a good camera set up but with the digital camera you can already make great images!

Wim
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piotr



Joined: 28 Apr 2004
Posts: 445
Location: San Diego

PostPosted: Tue Apr 12, 2005 9:30 am    Post subject: Reply with quote

Wim, thank you very much! I'm about to try a film camera and compare results.
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