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Paramecium dividing - in polarized light

 
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piotr



Joined: 28 Apr 2004
Posts: 445
Location: San Diego

PostPosted: Tue Mar 29, 2005 9:57 pm    Post subject: Paramecium dividing - in polarized light Reply with quote

This picture of Paramecium I took with crossed polars. The little birefringent crystals found inside a cell consist of uric acid, calcium oxalate, calcium carbonate, calcium phosphate, amino acids and other substances (Wichterman, 1953).



Here I added a home-made retardation plate:



25x/0.60 objective, Sony DSC-V1.
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Frez



Joined: 08 May 2004
Posts: 419
Location: New Hampshire USA

PostPosted: Tue Mar 29, 2005 10:16 pm    Post subject: Reply with quote

Oh that's beautiful! I'm blown away by this. All of those crystals...that's amazing. Very, very nice! Congrats!

Frez
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Kenv



Joined: 24 May 2004
Posts: 852
Location: New Zealand

PostPosted: Wed Mar 30, 2005 2:34 pm    Post subject: Reply with quote

Looks live a far-off galaxy photographed by the Hubble, beautiful Piotr.
Kenv
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Charles Krebs



Joined: 13 Jul 2004
Posts: 1200
Location: Issaquah, WA USA

PostPosted: Wed Mar 30, 2005 5:36 pm    Post subject: Reply with quote

Really nice piotr. Polarized light is amazing with a microscope, too bad it sucks up so much light. Imagine all the other things that are going on right under our eyes but were just not looking in the proper way to see them. You should try a few shots with the polarizers not quite as close to extinction as well. They are all interesting but different.
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piotr



Joined: 28 Apr 2004
Posts: 445
Location: San Diego

PostPosted: Wed Mar 30, 2005 6:09 pm    Post subject: Reply with quote

Thank you, guys! Smile

The first picture is a bit overprocessed, I will replace it later.

Charlie, yes, the effect with partially crossed polars is very nice, too. I will be doing more experiments.

Apparently - there are protozoans rich in micro-crystals, others don't include birefringent crystals at all. I think Vorticella is another interesting example.
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Ken Ramos
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Joined: 04 May 2004
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Location: Western North Carolina

PostPosted: Wed Mar 30, 2005 7:44 pm    Post subject: Reply with quote

I am all for the Vorticella Piotr. Lets see one. Awesome images here of the Paramecium, great captures. Very Happy
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Reposts of my images within the galleries are welcome, as are constructive critical critiques.
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Garry DeLong



Joined: 04 Apr 2005
Posts: 38
Location: Portland, Oregon USA

PostPosted: Tue Apr 12, 2005 10:35 am    Post subject: Reply with quote

Uric acid is especially beautiful. Its characteristic rainbow appearance under polarized light was useful for us medical lab types to confirm uric acid crystals in various lab specimens.
Garry DeLong
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