Here's an image of the whole moth brain section. I stitched 35 200x frames together to make this (using ImageAssembler). The resulting file is about 30MB. I printed a 3-foot long panorama from the stitch which shows tons of detail (mostly of the air tubes). Unfortunately, by the time I had time to take this picture sequence, most of the stain had bled out of the brain and into the glycerine. Too bad. Again, this is brightfield with a 20x phaco.
The brain is about 4mm across. The two large outer structures are the optic lobes, and we already talked about the antennae lobes. The U-shaped opening at the center is the esophagus! The billions of black fibers are air tubes supplying the oxygen.
Steve
moth brain stitch
Moderators: MacroMike, nzmacro, Ken Ramos, twebster, S. Alden
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- Location: Tucson, AZ USA
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- Posts: 545
- Joined: Sun Oct 10, 2004 9:23 pm
- Location: Tucson, AZ USA
Hi Frez,
Glad you like the moth brain
The stitching was a pain since there were so many threads of air tubes, the stitcher got all confused. I had to put manual control points in each frame connecting to the adjacent frames. If I recall, the stitcher worked about 2 hours working out the distortions, color blending, etc
I originally tried a single image with the Nikon 4x plan, but I couldn't produce good illumination over the 4mm area. Besides, the resolution was too low to see anything cool. So a 20x stitch was called for.
Steve
Glad you like the moth brain
The stitching was a pain since there were so many threads of air tubes, the stitcher got all confused. I had to put manual control points in each frame connecting to the adjacent frames. If I recall, the stitcher worked about 2 hours working out the distortions, color blending, etc
I originally tried a single image with the Nikon 4x plan, but I couldn't produce good illumination over the 4mm area. Besides, the resolution was too low to see anything cool. So a 20x stitch was called for.
Steve
- twebster
- Site Admin
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- Location: Phoenix "Valley of the Sun", Arizona, USA
Hi ya' Steve
Thank you so much for sharing this with us. What a wonderful image and such seamless stitching. That's what makes it our Administrator's Appreciation image for the months of December/January. Great job, Steve
All the best, my friend
Thank you so much for sharing this with us. What a wonderful image and such seamless stitching. That's what makes it our Administrator's Appreciation image for the months of December/January. Great job, Steve
All the best, my friend
Tom Webster
Administrator
Phoenix "The Valley of the Sun", Arizona, USA
Think about this...maybe Murphy is an optimist!!!
Administrator
Phoenix "The Valley of the Sun", Arizona, USA
Think about this...maybe Murphy is an optimist!!!
Congrats Steve, that is a great shot and some excellent work. I have never tried "stitching" images .
Site Admin.
Kenneth Ramos
Rutherfordton, North Carolina
Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
Kenneth Ramos
Rutherfordton, North Carolina
Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
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- Posts: 545
- Joined: Sun Oct 10, 2004 9:23 pm
- Location: Tucson, AZ USA